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Image Search Results
Journal:
Article Title: Elevated Levels of Circulating Interleukin-18 in Human Immunodeficiency Virus-Infected Individuals: Role of Peripheral Blood Mononuclear Cells and Implications for AIDS Pathogenesis
doi: 10.1128/JVI.76.24.12448-12456.2002
Figure Lengend Snippet: Effect of TGF-β on IL-18 production from PBMC. PBMC (2 × 105) from HIV-seronegative donors were cultured in 200 μl of the culture medium in 96-well plate alone or in the presence of recombinant human (rh) TGF-β (20 ng per ml; R & D Systems), TGF-β-neutralizing, or control antibody (5 μg per ml each; R & D Systems). Culture supernatants were collected 24 h later and assayed for IL-18 content with the ELISA kit. The figure shows average ± standard error IL-18 contents from three replicate wells. Only the addition of the neutralizing antibody resulted in a significant (P < 0.05) increase in TGF-β production.
Article Snippet: PBMC (2 × 10 5 ) from HIV-seronegative donors were cultured in 200 μl of the culture medium in 96-well plate alone or in the presence of recombinant
Techniques: Cell Culture, Recombinant, Enzyme-linked Immunosorbent Assay
Journal:
Article Title: Elevated Levels of Circulating Interleukin-18 in Human Immunodeficiency Virus-Infected Individuals: Role of Peripheral Blood Mononuclear Cells and Implications for AIDS Pathogenesis
doi: 10.1128/JVI.76.24.12448-12456.2002
Figure Lengend Snippet: TGF-β contents of the plasma samples. The level of TGF-β was measured in the samples with a commercial ELISA kit. The figure depicts average ± standard error concentration of this cytokine in the samples after its activation (total TGF-β). N and P, HIV-seronegative healthy and HIV-infected donors, respectively. The star on the top of a bar indicates a significant (P < 0.05) difference between the two group means.
Article Snippet: PBMC (2 × 10 5 ) from HIV-seronegative donors were cultured in 200 μl of the culture medium in 96-well plate alone or in the presence of recombinant
Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Activation Assay, Infection
Journal:
Article Title: Elevated Levels of Circulating Interleukin-18 in Human Immunodeficiency Virus-Infected Individuals: Role of Peripheral Blood Mononuclear Cells and Implications for AIDS Pathogenesis
doi: 10.1128/JVI.76.24.12448-12456.2002
Figure Lengend Snippet: Correlation between the levels of TGF-β in plasma and IL-18 in serum of HIV-infected individuals. The figure depicts a significant negative correlation between these two parameters as determined by the Pearson's method.
Article Snippet: PBMC (2 × 10 5 ) from HIV-seronegative donors were cultured in 200 μl of the culture medium in 96-well plate alone or in the presence of recombinant
Techniques: Infection